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Goat Anti-STIM1 Antibody (EB07469)

Related Products: EBP07469 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07469 Goat Anti-STIM1 Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $392.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com
EB07469 1
EB07469 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: STIM1, stromal interaction molecule 1, D11S4896E, GOK
Official Symbol: STIM1
Accession Number(s): NP_003147.2; NP_001264890.1; NP_001264891.1
Human GeneID(s): 6786
Non-Human GeneID(s): 20866 (mouse), 361618 (rat)

Immunogen

Peptide with sequence C-YIQNRYSKEHMKK, from the internal region of the protein sequence according to NP_003147.2; NP_001264890.1; NP_001264891.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Successful use of this antibody was reported in Western blot showing an approx. 80kDa in Human platelets: PMID 18439569

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in WB on Human Platelets (80kDa) in the following paper:
López JJ, Jardín I, Bobe R, Pariente JA, Enouf J, Salido GM, Rosado JA.
STIM1 regulates acidic Ca2+ store refilling by interaction with SERCA3 in human platelets.
Biochem Pharmacol. 2008 Jun 1;75(11):2157-64.
PMID: 18439569

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