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Goat Anti-AKT2 Antibody (EB06772)

Related Products: EBP06772 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06772 Goat Anti-AKT2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Rat $392.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com
EB06772 1
EB06772 (1µg/ml) staining of Human Skeletal Muscle (A) and (0.75ug/ml) Human Testes (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06772 2
EB06772 (1µg/ml) staining of Rat Testes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: AKT2, v-akt murine thymoma viral oncogene homolog 2, HGNC:392, PKBBETA, PRKBB, RAC-BETA, Murine thymoma viral (v-akt) homolog-2, rac protein kinase beta
Official Symbol: AKT2
Accession Number(s): NP_001617.1; NP_001229956.1; NP_001317440.1
Human GeneID(s): 208
Important Comments: This antibody is expectesd to recognise all reported iasoforms NP_001617.1; NP_001229956.1; NP_001317440.1.

Immunogen

Peptide with sequence C-RYDSLGLLELDQRT, from the C Terminus of the protein sequence according to NP_001617.1; NP_001229956.1; NP_001317440.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 58-60kDa band observed in Human Skeletal Muscle lysates and in Rat Testis lysates, and approx.60kDa in Human Testis lysates (calculated MW of 55.8kDa according to Human NP_001617.1 and 55.5kDa according to Rat NP_058789.1). Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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